PDF Membrane Orientation and Lateral Diffusion of BODIPY-Cholesterol as a ... Two-photon time-lapse microscopy of BODIPY-cholesterol reveals ... Bodipy-LDL fluorescence is neither quenched at high concentration nor reduced by acidic pH as in the lysosomes to which LDL-bound LDLRs traffic, so it can be used to quantify intracellular LDL . The green signal represents Bodipy; the Hoechst nuclear staining is in blue. The domains of a cholesterol-dependent cytolysin undergo a major FRET ... A 08 angle corresponds to BODIPY-cholesterol standing upright in a manner where BODIPY is in the membrane center. (Invitrogen) following the manufacturer's protocol. C11 Bodipy 581/591实验说明|C11 Bodipy 581/591脂质过氧化荧光探针|Cas：217075-36-0 BODIPY or boron dipyrromethene is a class of strong ultraviolet-absorbing molecules demonstrating a relatively sharp emission peak. Results are based on five (endogenous cholesterol) six (BODIPY-cholesterol) independent samples and presented as the mean percent BODIPY-cholesterol or endogenous cholesterol remaining ± SEM. 1 A) and demonstrated that it partitions into the L o phase of bilayers. Di-4 ANEPPDHQ (Invitrogen) in dimethyl sulfoxide (DMSO) was added to a concentration of 3 µg/ml, and erythrocytes at ≈0.1 hematocrit were incubated for 30 min at RT. Optimization of BODIPY 505/515 staining 2.3.1 BODIPY 505/515 concentration. PFO domains and possible rearrangements. A novel fluorescent cholesterol analog, with a BODIPY moiety linked to carbon-24 of the cholesterol side chain (BODIPY-cholesterol or BODIPY-chol) was recently characterized in detail, both in vitro and in vivo 9-12. CholEsteryl 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-<i>s</i ... Because of its unique spectral properties and superior photo-stability compared to other fluorophores such as nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) and fluorescein, we subsequently generated other Bodipy-analogs for lipid trafficking studies. a cholesterol-Bodipy (Bdp-Chol) derivative (Fig. PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol ... . PDF Membrane Fluidity and Lipid Order in Ternary Giant Unilamellar Vesicles ... New BODIPY lipid probes for fluorescence studies of membranes Scale bar = 50 µm. Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) Combined Light and Electron Microscopy Using Diaminobenzidine ... Structures of Bodipy-cholesterol, Bodipy-cholesteryl oleate, and ...
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